Mapping the Genotype–Phenotype Relationship in Cellular Signaling Networks: Building Bridges Over the Unknown - Systems Biology: Networks, Models, and Applications - page 143

proteins with which the
target interacts); and the
mixture is washed, such
that the bound proteins are
eluted and analyzed.

Crosslinking Indicates (1) the structural Capable of identifying weak Often detects a neighboring [27-32]

characteristics of proteins interactions that are not protein that may not be in

or complexes isolated identified with other methods. direct contact with the target

from within a cell; and Transient contacts between of interest.

(2) which proteins interact proteins at various stages in

with a specific test target. a dynamic process may be

Proteins are covalently detected by crosslinking and

crosslinked with agents freezing the process at

that bind to reactive groups different time points.

of amino acid residues. Can be performed in vivo

The resulting complex is with membrane permeable

analyzed for protein crosslinking reagents.

interactions, interaction Is often combined with

sites, and residue contacts. sequence analysis for

improved results.

Microarray Can indicate the phenotypic Can be used to characterize a Results observed are dependent [9-12, 33-35]

function of a set of genes. complete cellular phenotype, on the elements present on the

A cDNA or oligonucleotide i.e., a profile of all the chip, to the extent that extensive

library is printed on a genes that are activated and homology or antisense

high-density microchip, and inactivated under certain transcripts may cause

fluorescently tagged mRNA cellular conditions. cross-hybridization.

Continued