Biomedical Engineering Reference
In-Depth Information
Fig. 4
Co-localizing of dif erent proteins in the same tissue or cell. Double immunol uorescence
staining for E-cadherin (red) and b-catenin (green) as seen with a laser scanning confocal
microscope. Reprinted from Schmetz
et al.
(2001), with permission.
Color image of this i gure appears in the color plate section at the end of the topic.
Fig. 5
Fluorescent immunohistochemistry of p120 in MLO-Y4 osteocytes using l uorescence
microscopy. Fluorescent immunohistochemistry for labeled antibodies against mouse-p120 in
MLO-Y4 cells. h e primary antibody was mouse anti-p120 (BD Bioscience) with an Alexa Fluor
488 goat anti-mouse secondary antibody (Invitrogen). Yellow dots of labeled p120 are found
scat ered evenly throughout the cytoplasm of the MLO-Y4 cell.
Color image of this i gure appears in the color plate section at the end of the topic.
