Bioinformatics Analysis to Identify Cell Adhesion Molecules in Cancer - Adhesion Molecules - page 312

Inject pool of phage display peptide Inject pool of phage display peptide Inject pool of phage display peptide

library intravenously into mice

Inject pool of phage display peptide
library intravenously into mice library intravenously into mice library intravenously into mice

Select organ-
specific phages

Select organ-
specific phages

Select organ Select organ

Amplify Amplify

Amplify Amplify

specific phages specific phages

Select Select

Select Select

Inject selected phages intravenously
into mice

Inject selected phages intravenously
into mice

Inject selected phages intravenously Inject selected phages intravenously

into mice into mice

Select and sequence DNA of clones Select and sequence DNA of clones Select and sequence DNA of clones

containing peptides

Select and sequence DNA of clones
containing peptides

containing peptides containing peptides

Organ Organ Organ- specific Organ- specific specific- homing specific- homing homing peptides homing peptides peptides peptides

Bioinformatics Analysis
using MCAM database

Bioinformatics Analysis
using MCAM database

Bioinformatics Analysis Bioinformatics Analysis

using MCAM database using MCAM database

Tumor Tumor Tumor - specific CAMs that interact with Tumor -

organ

specific CAMs that interact with
organ organ- specfic peptides/CAMs organ- specfic peptides/CAMs specfic peptides/CAMs specfic peptides/CAMs

Fig. 1 A representation of in vivo phage display peptide library screening and bioinformatics
analysis to i nd tumor-associated CAMs. A pool of phages containing seven amino acid peptides
was injected intravenously into mice. Dif erent organs were harvested at er 5 min to isolate, purify
and count organ-specii c phage. Selected phages were reinjected two times to avoid non-specii c
phage. h e DNA of the phage were cloned and sequenced to identify nucleotides corresponding
to organ-specii c peptides. Bioinformatics analysis of peptide sequences using MCAM database
resulted in tumor-specii c CAMs that bind to organ-specii c peptides or CAMs (Sadanandam et al .
2007, 2008a).

using simple microbiological and recombinant DNA procedures. h e number of
peptides that can be accommodated with this technology far exceeds that which is
achievable using conventional expression systems. Phage display peptide libraries
not only allow selective exploration of cell surface features of the tumor or organ-
specii c microenvironments, but also provide ligand-directed therapeutics to the
tumor surface (Kolonin et al . 2006). To this end, peptides identii ed using phage
display libraries are being used to identify tumor- or organ-specii c CAMs by
bioinformatics strategies.