Biomedical Engineering Reference
In-Depth Information
Fig. 5
Ef ect of OPG on adhesion molecule expression in TNF-a-activated HUVECs
transfected with control (i) or angiopoietin-2-targeted (ii) siRNA. HUVECs were transfected
with siRNA 48 hr prior to incubation with TNF-a (50 U/mL) and OPG (10 ng/mL) for 4 hr.
Gene expression of ICAM-1 (A), VCAM-1 (B), E-selectin (C), ICAM-2 (D) and angiopoietin-2
(E) was measured by MALDI-TOF MS. In control siRNA-transfected HUVECs, OPG induced
an upregulation in ICAM-1, VCAM-1, E-selectin, ICAM-2 and angiopoietin-2 expression (*p =
0.05). However, in endothelial cells transfected with angiopoietin-2-targeted siRNA, OPG alone
had no ef ect on adhesion molecule or angiopoietin-2 expression (p = 0.4-0.9). Reproduced with
permission from Mangan
et al.
(2007).
