Environmental Engineering Reference
In-Depth Information
TAble 6.4
MPN Reference Chart
Sample Volume (mL)
Sample Volume (mL)
10
1.0
0.1
MPN/100 mL
10
1.0
0.1
MPN/100 mL
0
0
0
0
4
2
0
22
0
0
1
2
4
2
1
26
0
1
0
2
4
3
0
27
0
2
0
4
4
3
1
33
4
4
0
34
1
0
0
2
1
0
1
4
5
0
0
23
1
1
0
4
5
0
1
31
1
1
1
6
5
0
2
43
1
2
0
6
5
1
0
33
5
1
1
46
2
0
0
5
5
1
2
63
2
0
1
7
5
2
0
49
2
1
0
7
5
2
1
70
2
1
1
9
5
2
2
94
2
2
0
9
5
3
0
79
2
3
0
12
5
3
1
110
5
3
2
140
3
0
0
8
5
3
3
180
3
0
1
11
5
4
0
130
3
1
0
11
5
4
1
170
3
1
1
14
5
4
2
220
3
2
0
14
5
4
3
280
3
2
1
17
5
4
4
350
5
5
0
240
4
0
0
13
5
5
1
350
4
0
1
17
5
5
2
540
4
1
0
17
5
5
3
920
4
1
1
21
5
5
4
1600
4
1
2
26
5
5
5
≥2400
Note: As related to the MF technique, the coliform group may be defined
as comprising aerobic and many facultative anaerobic, Gram-negative,
non-spore-forming, rod-shaped bacteria that develop red colonies with a
metallic sheen within 24 hours at 35°C on an Endo-type medium. Some
members may produce dark red, mucoid, or nucleated colonies without
a metallic sheen. When verified these are classified as atypical coliform
colonies. When purified cultures of coliform bacteria are tested, they
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