Agriculture Reference
In-Depth Information
Chenopodiaceae
Example: Beta vulgaris
1. Moistening: Soak in water 18 hr.
2. Preparation after moistening:
a. Remove one side or the back of the seed, including a thin slice of the embryo.
b. Puncture through the seed coat near the border of the embryo and the nutritive tissue.
3. Staining time: 24-48 hr.
4. Preparation for evaluation: Expose the embryo and adjoining nutritive tissue by spreading the cut
surfaces suficiently to expose the desired tissue.
5. Evaluation of viable seeds: Embryo should be completely stained except for a very small area of
the distal portion of the radicle and cotyledons.
Chenopodiaceae
Beta vulgaris (Beet)
(1)
(2)
(1)
(2)
Viable Seed
Non-Viable Seed
(1) Embryo entirely stained, and (2) green
embryo with irm tissues.
(1) Radicle or plumule are unstained, and
(2) green embryo with soft tissues.
 
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