Agriculture Reference
In-Depth Information
Pinaceae
Example: Pinus spp.
1. Preconditioning: Remove basal end of the seed, including a tip of the nutritive tissue.
2. Moistening: Soak in water 18 hr.
3. Preparation after moistening:
a. Additional seed coat preparation is not usually necessary, except to accelerate the uptake of
water and/or the staining solution.
b. Cut the seeds longitudinally through the seed coat and into the nutritive tissue along the entire
length near the midsection.
c. Cut the seeds longitudinally off-center through the seed coat and nutritive tissue to expose the
outline of the intact embryo.
d. Remove distal end of the radicle, including a fragment of the nutritive tissue.
4. Staining time: 24-48 hr.
5. Preparation for evaluation: Expose the embryo and the adjoining nutritive tissue by spreading the
cut surfaces suficiently to expose the desired tissue.
6. Evaluation of viable seeds: The embryo must be completely stained. Nutritive storage tissue apart
from embryo stained except for small surface necroses that are not in contact with embryo cavity.
Pinaceae
Pinus spp. (Pine)
(1)
(2)
(1)
(2)
Viable Seed
Non-Viable Seed
(1) Embryo is entirely stained, and (2)
small unstained areas of the megagame-
tophyte (i.e., haploid storage tissues in
gymnosperm).
(1) Embryonic axis and/or cotyledons
unstained, and (2) unstained megagameto-
phyte.
 
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