Biomedical Engineering Reference
In-Depth Information
4.4
Online Resources
The internet has a large volume of literature on fluorescence microscopy, deconvo-
lution and softwares for analysis and visualization. While it is difficult to list them
all, we have selected those that we consider relevant to this chapter.
4.4.1 Microscopy Primers
There are many online tutorials and demonstrations from the microscopy commu-
nity which gives an idea of the working of the WFM and the CLSM.
Virtual Java Environment on WFM and CLSM
http://www.microscopyu.com/tutorials/java/virtual/confocal/index.html
http://www.olympusmicro.com/primer/virtual/confocal/index.html
From the given list, the user can select the specimen to image and the applet gives
an impression of working with real microscope settings. The parameters that one
can play with are the focusing into the samples, adjusting the other microscope
parameters like the brightness, gain, and the scan speed.
4.4.2 Databases
There are also a few online sites where confocal images could be downloaded for
visualization and computational analysis. The users may browse the data online
or download it for reuse within the guidelines of each of the following site usage
agreement. Here is a list of few of them.
Images used in this chapter
http://www-syscom.univ-mlv.fr/ANRDIAMOND/images.html
The images used in this chapter can be downloaded from the ANR DIAMOND
project. These are primarily from a Zeiss LSM 510 confocal microscope and can be
visualized using the softwares listed in the next section.
Simulating fluorescence microscopy images (CBIA, Masaryk University)
http://cbia.fi.muni.cz/simulator
The centre for biomedical image analysis (CBIA) also provides an on-line frame-
work for simulating fluorescence microscopy images.
Images from BIG, EPFL
http://bigwww.epfl.ch/deconvolution/?p=bio
The EPFL Biomedical imaging group (BIG) have also a few trial images that are
made available for validating the algorithms.
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