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layer was observed between the coating and cell layer (denoted
by d in Fig. 6.27a) but it was not found from the surface of CaPvir
coating (Fig. 6.27c). After culturing for 16 days, a calcified layer was
formed of both coatings (marked by d in Figs. 6.27b, d). It induced
the formation of an interface between the coating and mineralised
matrix. This layer on the CaPpre coating was much thicker than that
on the CaPvir coating. In addition, the network of collagen fibers of
the ECM could be observed together with globular CaP accretions
(denoted by m). The directly bonded layer was dense whereas, the
accretions were globular, not interconnected, and formed in the
collagen fiber network.
Figure 6.27
Side view SEM micrographs after culturing for 8 and 16 days.
Top row: pre-immersed CaP in SBF (CaPpre) after (a) 8 days
and (b) 16 days. Bottom row: CaPvir (untreated CaP) after (c)
8 days and (d) 16 days. Magnification: 10,000
. Annotations:
s, substrate; b, cell blanket; d, directly bonded CaP layer; m,
globular CaP accretions and collagen matrix. Reprinted from
Ref. [58], Copyright 2006, with permission from Elsevier.
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