Biomedical Engineering Reference
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IID TATA binding protein, transferring receptor, and 18S
rRNA. For a good control gene, the stress treatment should
not change the gene expression compared to that in unstressed
cells. We found that the expression of eight of the genes
changed in response to the stress treatment. Among the
remaining three genes that appeared unaffected by the stress
treatment, we selected b-actin as endogenous control gene.
8. You can buy pre-developed TaqMan
®
gene expression
assays from Applied Biosystems or you can design it yourself.
If you choose to design the assays yourself it is a very good
idea to define the sequence of the probe to span an exon-
exon junction. In this way, you exclude any possible ampli-
fication of genomic DNA which may be present in the RNA
preparation.
Acknowledgements
The authors would like to thank lector Thomas J. Corydon at the
Institute for Human Genetics, University of Aarhus, for advice,
support and use of cell culturing facilities. This work has been
supported by The Danish Medical Research Council; Aarhus
University Hospital's Research Initiative; Institute of experimen-
tal Clinical Research, University of Aarhus; Faculty of Health
Sciences, University of Aarhus.
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