Biomedical Engineering Reference
In-Depth Information
mRNA expression
(relative to
b
-actin mRNA)
Protein expression
(relative to total soluble fibroblast protein)
5
70
HO1
Hsp70
HO1
Hsp70
60
4
50
3
40
Heat shock
30
2
20
1
10
0
0
0
2
4
6
8
10
12 14
16
18
20
22
24
26
0
2
4
6
8
10
12
14
16
18
20
22
24
26
Recovery time [hours]
Recovery time [hours]
30
14
HO1
Hsp70
HO1
Hsp70
12
25
10
20
20
8
Oxidative
stress
15
6
10
4
5
2
0
0
0
2
4
6
8
10
12
14
16
18
20
22
24
26
0
2
4
6
8
10
12
14
16
18
20
22
24
26
Recovery time [hours]
Recovery time [hours]
5
5
HO1
Hsp70
HO1
Hsp70
4
4
3
3
No stress
2
2
1
1
0
0
0
2
4
6
8
10
12
14
16
18
20
22
24
26
0
2
4
6
8
10
12
14
16
18
20
22
24
26
Recovery time [hours]
Recovery time [hours]
Fig. 2. Stress response proiles in human ibroblasts. Hsp70 and HO1 mRNA and protein expression proiles in human ibroblasts exposed to heat shock (44°C), oxidative stress
(600 mM H
2
O
2
), or no stress for 1 h followed by 24 h recovery at 37°C. The expression of Hsp70 and HO1 mRNA is measured relative to
b
-actin mRNA, while Hsp70 and HO1 protein
expressions are measured relative to total soluble ibroblast protein. All expression proiles are normalized to unstressed cells at 24 h “recovery”. The
curves
represent mean curves
of two independent experiments and the standard deviation is estimated according to: SD =
√
(
S
d
2
/2N), d = difference between the two experiments; N = number of differences. Note
different scales on the axes. —
■
— (Hsp70); —
●
— (HO1).
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