Biomedical Engineering Reference
In-Depth Information
Table 1
Details of TaqMan gene expression assays for QPCR
Assay
Forward primer
Reverse primer
Probe (exon-exon border)
a
Fluorescence
b
Hsp70
(Hs00271244_s1)
a
No information
No information
No information (no introns)
6-FAM
TM
(NFQ)
HO1
5¢-CCAGCGGGCCAGCAA-3¢ 5¢-GGGAGCGGGTGTTGAGT-3¢ 5¢-CAAAGTGCAAGATTCTG-3¢(4-5)
6-FAM
TM
(NFQ)
b-actin (4310881E)
a
No information
No information
No information
VIC
TM
(TAMRA
TM
)
a
ID number for a predeveloped assay designed by Applied Biosystems; primer/probe sequences are not available
b
The probes are conjugated to a reporter dye (6-FAM™ or VIC™) in the 5¢-end and to a quencher (non-luorescent (NFQ) or TAMRA™) in the 3¢-end
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