Biomedical Engineering Reference
In-Depth Information
(a) Target DNA (complementary)
5'CCCCCCCTCCCggg
A
G
AgCCATAgTggT3'
(b) 1 base mismatched DNA
5'CCCCCCCTCCCggg
A
A
AgCCATAgTggT3'
60
50
(a)
40
30
(b)
20
10
0
300
400
500
600
700
800
E vs. Ag /AgCl (mV)
Fig. 4.12.
Differential pulse voltammograms of Hoechst 33258 on HCV 1a probe-
modified electrode reacted with 100 nM target DNA (complementary) or one base
mismatched DNA at 100 mV/s. Electrode area 200
×
200
m
2
μ
intercalator
injection
sample
injection
PCR
sensor
PCR
chamber
Fig. 4.13.
The structure of the PCR chip
was transferred onto the glass substrates using a photoresist, a photomask,
and UV exposure, and the chip was coated in a photoresist. The chamber
plate was also transferred onto the substrate using same techniques, and
etched into the substrate in dilute HF/HNO
3
. Then, the chip was covered
with the chamber plate. This structure is simple and versatile, enabling easy
accommodation of different chip designs. This kind of integration is not di-
