Synthetic Dicer-Substrate siRNAs as Triggers of RNA Interference - RNA Interference from Biology to Therapeutics - page 38

Fig. 2.4 Comparison of potency of an anti- TP53 DsiRNA and the cognate siRNA. ( a ) An asym-
metric 25/27-nt DsiRNA and its cognate 21-nt siRNA targeting TP53 (NM_000546) were trans-
fected into HeLa cells at the indicated concentrations. RNA was isolated at 24 h post-transfection,
and RT-qPCR was performed to measure relative TP53 expression levels normalized to an internal
control gene, GAPDH . NC = negative control. ( b ) The same DsiRNA and siRNA were transfected
into HeLa cells, and cultures were maintained for 1-6 days as indicated. RNA was isolated, and
RT-qPCR was performed to measure TP53 expression levels as before. ( c ) Sequences of the
dsRNAs are shown; RNA bases are uppercase and DNA bases are lowercase . “p” indicates a
5¢-phosphate. Adapted from Hefner et al. [ 37 ] with permission from the Association of Biomolecular
Resource Facilities (ABRF)