Genome-Wide RNAi Screening to Identify Human Host Factors Crucial for In fl uenza Virus Replication - RNA Interference from Biology to Therapeutics - page 247

Table 12.1 RNAi screens performed for the identification of human host factors associated in IAV infection and replication, as described in [ 4, 23- 25, 27 ] and
adapted from [ 5, 6, 28 ]

Karlas et al. [ 4 ] König et al. [ 25 ] Brass et al. [ 24 ] Hao et al. [ 23 ] Shapira et al. [ 27 ]

Cell type Human lung (A549) Human lung (A549) Human osteosarcoma

(U2OS)

Drosophila (DL1) Human (HBEC)

Virus strain WSN Recombinant WSN PR8 Recombinant WSN PR8

Readout method NP expression and

luciferase activity

Luciferase activity HA expression on

cell surface

Luciferase activity Infectious virus yield by

luciferase activity

Events in viral life cycle
covered by screen

Attachment until
budding/release

Attachment until
protein expression

Attachment until HA
surface traf fi cking

Uncoating until protein
expression

Attachment until
budding/release

siRNA library used Qiagen Qiagen Dharmacon Ambion Dharmacon

Number of genes targeted 22,843 19,628 17,877 13,071 1,745 a

Number of fi ltered hits 287 295 312 110 Not determined

a 1,745 candidate genes identified via Y2H and genome-wide microarray analyses subjected to siRNA screen for validation