Agriculture Reference
In-Depth Information
Table 10.3
Summary of
in vivo
probiotic applications on sole species.
Probiont
Parameters investigated
References
Senegalese sole
Vibrio
sp. (2J18), Gram-positive sp.
(J84),
Shewanella
sp. (2J27)
GM, SR
Makridis
et al
. (2008)
Sh. putrefaciens
(Pdp11) and
Sh.
baltica
(Pdp13)
BC, D-EA, GH, GM, GP, SR
Sáenz de Rodrigáñez
et al
.
(2009)
Sh. putrefaciens
(Pdp11) and
Sh.
baltica
(Pdp13)
DR, GP, IR
Díaz-Rosales
et al
. (2009)
Sh. putrefaciens
(Pdp11) and
Sh.
baltica
(Pdp13)
BC, DR, GH, GM, GP, SR
de La Banda
et al
. (2010)
Sh. putrefaciens
(Pdp11)
BC, DR, GP
de La Banda
et al
. (2012)
Sh. putrefaciens
(Pdp11)
GM
Tapia-Paniagua
et al
.
(2010)
Sh. putrefaciens
(Pdp11)
BC, GM, GP, SR
Lobo
et al
. (2013)
Common sole
E. faecium
IMC 511
GP, GM, SR, STR
Avella
et al
. (2011)
E. faecium
IMC 511
GP, STR
Palermo
et al
. (2011)
Source: Adapted from Dimitroglou
et al
. 2011.
Genera abbreviations:
E
.
=
Enterococcus
,
Sh
.
=
Shewanella
.
Parameters investigated: BC
=
body composition, D-EA
=
digestive/intestinal enzymes, DR
=
disease resistance, GH
=
gut
histology/immunohistochemistry, GM
=
gut microbiota (inclusive of GI probiont recovery), GP
=
growth performance,
IR
=
immunological/haematological response, SR
=
survival rate, STR
=
stress tolerance/response/biomarkers.
2010); and common or Dover sole (
Soleasolea
). A number of probiotics have been isolated and
tested on sole and several strains isolated from different teleost species have also been applied
(Dimitroglou
et al
. 2011; Table 10.3). In particular, the probiotic strains Pdp11 and Pdp13,
from the Alteromonadaceae family, are among the most well researched Gram-negative bac-
terial probiotics in fish (Díaz-Rosales
et al
. 2006; 2009; Sáenz de Rodrigáñez
et al
. 2009; de
La Banda
et al
. 2010; Tapia-Paniagua
et al
. 2010; 2012). These strains were originally iso-
lated from gilthead sea bream skin and later identified as
Shewanella putrefaciens
(Pdp11)
and
Shewanella baltica
(Pdp13) (Díaz-Rosales
et al
. 2009). These probiotic species have been
extensively studied in Senegalese sole and varying benefits have been documented for growth
performance, immunomodulation and disease resistance (for a review see Tapia-Paniagua
etal
.
2012). These strains have been reported to modulate the gut microbiota of sole by changing
operational taxonomic units (OTU) community profiles, elevating inter-fish community sim-
ilarities and elevating the abundance of species related to the
Vibrio
genus; readers with a
specific interest on their abilities to modulate the gut microbiota are referred to
Chapter 8
.
10.4.1 Effects on growth
Sáenz de Rodrigáñez and collaborators (2009) administered dietary supplementation of
Sh.
putrefaciens
(Pdp11) and
Sh. baltica
(Pdp13) to juvenile Senegalese sole for 60 days. Experi-
mental diets were made in the laboratory by supplementation of the diet with lyophilized cells
of Pdp11 and Pdp13 (at 10
9
CFU g
−1
) in an alginate based top coat. The dose was the same as
previous studies which evidenced health benefits in
S.senegalensis
(Díaz-Rosales
etal
. 2009).
The controls were fed only with a probiotic-free alginate top coated diet. Growth and nutrient
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