Probiotic Applications in Temperate and Warm Water Fish Species - Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics - page 259

Table 10.2 Summary of in vivo gilthead sea bream probiotic studies.

Probiont Parameters

investigated

References

Lb. delbrueckii subsp. lactis , B. subtilis IR Salinas et al . (2005)

Lb. delbrueckii subsp. lactis , B. subtilis IR Salinas et al . (2008)

Lb. fructivorans and Lb. plantarum GM, SR Carnevali et al . (2004)

Lb. fructivorans , Lb. plantarum GM, SR, STR Rollo et al . (2006)

Lb. fructivorans , Lb. plantarum GH, IR Picchietti et al . (2007)

Lb. delbrueckii subsp. delbrueckii , Lb.
fructivorans and Lb. plantarum

GP, IR Abelli et al . (2009)

Vagococcus fluvialis IR Romàn et al . (2012)

Commercial product containing: Lb.
plantarum , Lb. bulgaricus , Lb. acidophilus ,
Lb. rhamnosus , Str. thermophilus , Bi.
bifidum , E. faecium

D-EA,GM,GP,SR Suzer et al . (2008)

Sh. putrefaciens (Pdp11), 51M6
( Shewanella sp.), L. delbrueckii , B. subtilis

IR Salinas et al . (2006)

Cytophaga spp., Roseobacter spp.,
Ruegeria spp., Paracoccus spp.,
Aeromonas spp., Shewanella spp.

SR Makridis et al . (2005)

Sh. putrefaciens (Pdp11), 51M6
( Shewanella sp.), and Micrococcus sp.
(Pdp5)

MA, DR Chabrillón et al . (2006)

Sh. putrefaciens (Pdp11) and 51M6
( Shewanella spp.)

IR Díaz-Rosales et al . (2006)

Sh. putrefaciens BC, GH, GP, IR, STR Varela et al . (2010)

B. subtilis , Tetraselmis chuii and
Phaeodactylum tricornutum

DR, IR Cerezuela et al . (2012a)

B. subtilis , Tetraselmis chuii and
Phaeodactylum tricornutum

GH, GM Cerezuela et al . (2012b)

D. hansenii AO-EA, IR, MA Reyes-Becerril et al .

(2008a)

D. hansenii GM, IR Tapia-Paniagua et al.

(2011)

D. hansenii GM, IR Reyes-Becerril et al . (2012)

Source: Adapted from Dimitroglou et al . 2011.
Genera abbreviations: B . = Bacillus , Bi = Bifidobacterium , D . = Debaryomyces , E . = Enterococcus , Lb . = Lactobacillus ,
S . = Saccharomyces , Sh . = Shewanella , Str . = Streptococcus .
Parameters investigated: AO-EA = antioxidant enzymes, BC = body composition, D-EA = digestive/intestinal enzymes,
DR = disease resistance, GH = gut histology/immunohistochemistry, GM = gut microbiota (inclusive of GI probiont recovery),
GP = growth performance, IR = immunological/haematological response, MA = mucus adhesion, SR = survival rate,
STR = stress tolerance/response/biomarkers.

Growth (standard length and body weight) significantly increased in both experimental groups,
at both larval (47 dph) and juvenile (75 dph) stages. The increases in body weight and total
length were in agreement with the expression of genes involved in growth metabolism (IGFI
and myostatin) and in animal welfare (HSP70). The higher expression of IGFI and the lower
levels of myostatin in groups fed probiotics were concomitant with the lower expression of
HSP70 and glucocorticoid receptor (GR), evidencing the beneficial role of the supplied Bacil-
lus mixture on fish welfare; however, no differences in survival rate were found among groups
(Avella et al . 2010b).