Probiotic Applications in Cold Water Fish Species - Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics - page 242

Table 9.4 Probiotic applications in Pleuronectiformes and the main effects observed.

Rearing stage Probiotic used Administration mode Main effects observed Reference

Atlantic halibut ( Hippoglossus hippoglossus )
Larval
(32 days)

C. divergens -like ( Lactobacillus
plantarum )or Aliivibrio
( Vibrio ) salmonicida (ALL)

Ova and resulting larvae
incubated in RW containing
bacteria (10 6 CFUml -1 )

Significantly higher survival
32 dph compared to control

Ottesen and Olafsen
(2000)

Ova
(14 days)
Larval
(60 days)

Vibrio sp., V. splendidus ,and
Pseudoalteromonas elyakovii
(AUT)

Ova treated three times in
incubators (10 1-2 CFUml -1 )
and once (10 6 CFUml -1 ,5min)
after disinfection prior to hatch;
Artemia treated in bacterial
mixture (10 6 CFUml -1 )at30 ∘ C
for 30min (1/3 as morning
ration); the rest used as a
second ration (8 h later), treated
again in 10 4 CFUml -1

Ova treatment had no effect on
ova survival
Larval treatment performed four
times (days 0, 1, 17, 18) led to
higher survival, and sometimes
enhanced growth

Bjornsdottir et al. (2010)

Turbot ( Scophthalmus maximus )
Larval (20 days) Daily addition of 10 7 CFUml -1

rotifer culture for 6-7 days;
feeding trial of turbot larvae for
20 days

Lb. plantarum , Lb. helveticus
or Str. thermophilus (ALL)

Lb. plantarum enhanced the rotifer
production rate significantly and
reduced their bacterial load; all
LAB fed larvae were heavier
than control; no increase in
survival

Gatesoupe (1991)

(10 days) Bacillus sp. IP 5832 (Paciflor

9) (ALL)

Bacillus -enriched rotifers (4 ×
10 3 CFUml -1 per day); larval
feeding

Bacillus treatment of rotifers for 5
days led to microbiota
modulation; significant growth
and survival increase after 10
days in Bacillus -fed turbot larvae

Gatesoupe (1993)

(9 days) Carnobacterium -like sp. (ALL) LAB-enriched rotifers; larvae

challenged with Vibrio sp.
(10 6 CFUml -1 , 9 dph)

No effect on growth or survival
under normal conditions;
increased survival upon Vibrio
challenge. LAB dose effect
observed (10 7 CFUml -1 )

Gatesoupe (1994)