The Effects of Kisspeptin on Gonadotropin Release in Non-human Mammals - Kisspeptin Signaling in Reproductive Biology - page 65

Fig. 4.1 Effect of central ( a ) or intravenous ( b ) administration of 77 nmol of kisspeptin-10 ( fi lled
circle ), vehicle ( fi lled triangle ), or 77 nmol of kisspeptin-10 with acyline pre-treatment ( open circle )
on plasma LH levels (mean ± SEM) in GnRH primed agonadal juvenile male monkeys. Kisspeptin
was injected at time 0 ( arrow ) and elicited robust LH secretion following both central and periph-
eral administration. The LH-releasing action of ICV or IV kisspeptin was abolished by pre-treat-
ment with acyline. From Shahab et al. [ 9 ]. Reprinted with permission from National Academy of
Sciences, U.S.A. Copyright (2005)
bolus of kisspeptin-10 at 100 or 200 pmol/kg, with or without additional sex ste-
roids, signifi cantly increases plasma LH levels [ 12 ]. The effect of an IV bolus of
murine kisspeptin-10 (1 mg; 768 nmol) was also examined in light horse mares
(3-15 years of age) during diestrus (when endogenous LH and FSH are low). LH
levels increased by ~2-fold within 20-30 min of IV injection, returning to baseline
within 4 h [ 13 ].
Acute Intraperitoneal Administration
In 2004, Thompson and colleagues observed that intraperitoneal (IP) injections of
10, 30, and 100 nmol of kisspeptin-10 in male Wistar rats dose-dependently increased
plasma LH at 20 min, although only signifi cantly following the highest dose (LH
0.6 ng/mL after saline vs. 5.9 ng/mL after 100 nmol kisspeptin) (Table 4.1 ) [ 4 ].