Biology Reference
In-Depth Information
Table 22.1
Summary of trans
genic lines
Mouse line
Type of mutation
Major phenotype
Comments
References
Genetic modifi cation of Kiss1r/Gpr54 locus
Kiss1r
tm1Coll
Deletion of parts of exon 1 and 2
Hypogonadotrophic
Null mutation
[
26
]
Hypogonadism
Kiss1r
expression tagged with
LacZ
Kiss1r
tm1Gstn
Deletion of part of exon 2
Hypogonadotrophic
Null mutation
[
27
]
Hypogonadism
Kiss1r
expression tagged with
LacZ
Kiss1r
tm1Rla
Complete deletion of exon 2
Hypogonadotrophic
Null mutation
[
29
]
Hypogonadism
No reporter gene present
Kiss1r
Gt1Stei
Retroviral insertion intron 2. No loss
of coding sequence
Hypogonadotrophic
May be a hypomorphic mutation
[
30
,
31
]
Hypogonadism
Females can be induced to show a modest
pre-ovulatory LH surge
May retain low level expression of
Kiss1r
Kiss1r
tm1.1On
Deletion of all coding exons after a
CRE-mediated recombination step
Hypogonadotrophic
Null mutation
[
28
]
Hypogonadism
Kiss1r
expression tagged with NLS-
LacZ
Kiss1r
tm1.1 ( cre ) Uboe
IRES-CRE inserted just downstream
of coding sequence in exon 5
Fertile
Neutral mutation
. Kiss1r
still expressed
[
39
]
Kiss1r
expression tagged with CRE
Genetic modifi cation of Kiss1 locus
Kiss1
tm1Coll
Complete deletion of exon 1 and 2
Hypogonadotrophic
Null mutation
[
32
]
Hypogonadism
Kiss1
expression tagged with
LacZ
Kiss1
tm1Rla
Deletion of exon 1
Hypogonadotrophic
Null mutation
[
29
]
Hypogonadism
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