Biology Reference
In-Depth Information
Table 22.1
Summary of trans genic lines
Mouse line
Type of mutation
Major phenotype
Comments
References
Genetic modifi cation of Kiss1r/Gpr54 locus
Kiss1r tm1Coll
Deletion of parts of exon 1 and 2
Hypogonadotrophic
Null mutation
[ 26 ]
Hypogonadism
Kiss1r expression tagged with LacZ
Kiss1r tm1Gstn
Deletion of part of exon 2
Hypogonadotrophic
Null mutation
[ 27 ]
Hypogonadism
Kiss1r expression tagged with LacZ
Kiss1r tm1Rla
Complete deletion of exon 2
Hypogonadotrophic
Null mutation
[ 29 ]
Hypogonadism
No reporter gene present
Kiss1r Gt1Stei
Retroviral insertion intron 2. No loss
of coding sequence
Hypogonadotrophic
May be a hypomorphic mutation
[ 30 , 31 ]
Hypogonadism
Females can be induced to show a modest
pre-ovulatory LH surge
May retain low level expression of Kiss1r
Kiss1r tm1.1On
Deletion of all coding exons after a
CRE-mediated recombination step
Hypogonadotrophic
Null mutation
[ 28 ]
Hypogonadism
Kiss1r expression tagged with NLS- LacZ
Kiss1r tm1.1 ( cre ) Uboe
IRES-CRE inserted just downstream
of coding sequence in exon 5
Fertile
Neutral mutation . Kiss1r still expressed
[ 39 ]
Kiss1r expression tagged with CRE
Genetic modifi cation of Kiss1 locus
Kiss1 tm1Coll
Complete deletion of exon 1 and 2
Hypogonadotrophic
Null mutation
[ 32 ]
Hypogonadism
Kiss1 expression tagged with LacZ
Kiss1 tm1Rla
Deletion of exon 1
Hypogonadotrophic
Null mutation
[ 29 ]
Hypogonadism
 
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