Kisspeptin Excitation of GnRH Neurons - Kisspeptin Signaling in Reproductive Biology - page 113

Fig. 6.1 Kisspeptin depolarizes GnRH neurons in a concentration-dependent manner.
( a ) Representative traces showing that kisspeptin (1-100 nM) depolarized GnRH neurons in a
concentration-dependent manner. The initial membrane potential for each trace is indicated. Only
one cell was recorded from one slice. ( b ) Concentration-response curve of the kisspeptin-induced
depolarization. Data are presented as mean ± SEM. The EC 50 for the kisspeptin-induced depolari-
zation was 2.8 ± 0.2 nM ( n = 8-14) based on a logistic equation fi t to the data points. ( c ) The kiss-
peptin (10 nM)-induced depolarization was long lasting and typically took 30 min to recover. From
Zhang C, Roepke TA, Kelly MJ, Rønnekleiv OK. Kisspeptin depolarizes gonadotropin-releasing
hormone neurons through activation of TRPC-like cationic channels. J Neurosci 2008; 28: 4423-
4434. Reprinted with permission from The Society for Neuroscience
neurons responding to kisspeptin and proposed that there are two physiologically
distinct populations of GFP-GnRH neurons, one that responds to kisspeptin and
the other that responds to the metabotropic glutamate receptor agonist,
dihydroxyphenylglycine (DHPG) [ 51 ]. One explanation for these differences is that