Nanosensors - Nanotechnologies for Water Environment Applications - page 424

Table 13.2 Sensitivity of fluorophore and nanoparticle labeling-based microbial
detection assays.
Scheme description

Detection limit Reference

3.01 × 10 9 copies
of target gene /mL

DNA hybridization on glass slide; Cyanine3
labeling and fluorescent signaling; imaged by
confocal microscopy.

(Taton et al., 2000)

3.01 × 10 7 copies of
synthetic target gene
/mL

Sandwich-structure DNA hybridization for
target recognition; Gold nanoparticle labeling
for target identification; Silver enhancement;
gray level signaling of silver coating.

(Taton et al., 2000)

1.20 × 10 7 copies of
synthetic target gene
/mL

Sandwich-structure, DNA/RNA hybridization
for target recognition; Gold nanoparticle
labeling for target identification; Ag
enhancement; Surface-enhanced Raman
scattering signaling.

(Cao et al., 2002)

1.81 × 10 4 copies of
PSA /mL

Bar-code amplification (BCA); sandwich-
structure hybridization for target protein
(prostate-specific antigen) recognition; Au
nanoparticle labeling for target identification;
sandwich-structure hybridization for bar-code
DNA detection; Ag enhancement; gray level
signaling of silver coating.

(Nam et al., 2003)

3.01 × 10 2 copies of
functional gene /mL

BCA; sandwich-structure hybridization of target
DNA recognition; Gold nanoparticle labeling
for target DNA identification; sandwich-
structure hybridization for bar-code DNA
recognition; Silver enhancement; gray level
signaling of silver coating.

(Nam et al., 2004)

4.82 × 10 7 copies of
synthetic DNA
sequences/mL

Tetramethylrhodamine-doped silica nanoparticle
labeling of synthetic target DNA sequence;
sandwich DNA hybridization for target
recognition; TMR fluorescent signaling.

(Zhao et al., 2003)

~2.35 × 10 5 copies of
gDNA/mL

RuBpy-doped silica NP labeling of target
genomic DNA; sandwich DNA microarray for
target recognition; RuBpy fluorescent signaling.

(Lian et al., 2004)

RuBpy-doped silica nanoparticle labeling;
immunological chemistry (mAbs labeling);
Rubpy fluorescent signaling.

2.5 cells/mL (Zhao et al., 2004)