Biology Reference
In-Depth Information
Chapter 5
Sheep Calcaneal Tendon Repair Subsequent to
Amniotic-Derived Stem Cell Allotransplantation
V. Russo, M. Marchisio, L. Pierdomenico, B. Barboni, A. Ioannoni, L.
Valbonetti, A. Muttini, O. Di Giacinto, D. Nardinocchi, A. Mauro,
A. Martelli, R. Peli, and P. Berardinelli
Abstract This preclinical study was performed to evaluate the regenerative
capacities of vitro-expanded amniotic fluid stem cells (AFSCs) allotransplanted
within experimentally induced lesions in ovine calcaneal tendons. Explanted
samples were evaluated for tendon architecture, collagen composition, AFSC
retrieval, cell proliferation, blood vessel organization, and leukocyte infiltration.
Tendon healing and viable AFSCs were observed within the lesion site 30 days after
transplantation. AFSCs differentiated into collagen-producing cells, stimulated
collagen type I deposition and fiber alignment, and exerted positive effects on
angiogenesis and inflammatory reactions. Thus, as AFSCs markedly improved
tendon healing, they may be used to develop innovative therapies.
Keywords Allotransplantation • Amniotic fluid stem cells • Calcaneal tendon •
Sheep
V. Russo (
*
) • B. Barboni • A. Mauro
Dipartimento di Scienze Biomediche Comparate, University of Teramo, Teramo, Italy
e-mail:
vrusso@unite.it
M. Marchisio • L. Pierdomenico
Dipartimento di Scienze Biomediche Comparate, University of Teramo, Teramo, Italy
Dipartimento di Biomorfologia, University of Chieti-Pescara “G. D'Annunzio”, Chieti, Italy
A. Ioannoni • O. Di Giacinto • D. Nardinocchi • A. Martelli • R. Peli • P. Berardinelli
Dipartimento di Scienze Biomediche Comparate, University of Teramo, Teramo, Italy
L. Valbonetti
Dipartimento di Scienze Cliniche Veterinarie, University of Teramo, Teramo, Italy
A. Muttini
Dipartimento di Scienze Biomediche Comparate, University of Teramo, Teramo, Italy
Dipartimento di Scienze Cliniche Veterinarie, University of Teramo, Teramo, Italy
