Biology Reference
In-Depth Information
Chapter 33
Influence of Sample Storage on the Quality
of DNA Extracted from Milk of Goats Fed
Conventional or Transgenic Soybean
R. Tudisco, S. Calabr
`
, M.I. Cutrignelli, M. Grossi, V. Piccolo, and F. Infascelli
Abstract PCR approach was used in order to investigate the presence of plant DNA
in pellet of somatic cells from fresh or stored milk of twenty grazing goats fed
concentrate (18% CP; 1.03 UFL/kg, as fed) containing conventional or genetically
modified soybean meal. The quantity and the purity of DNAs extracted from fresh
samples were higher than those of samples stored at
20
C(ng/
l: 51.6
vs
20.8;
A
260
/A
280
:1.7
vs
1.4). The frequency of chloroplast sequence, for both groups, ranged
between 70 and 100% in fresh and between 30 and 40% in stored samples. The
frequency percentage of specific soybean gene was lower in stored (25-33%) than in
fresh samples (56-88%). Transgenic fragments were found only in milk from goats
fed GM soybean. For 35S promoter, the frequency was 38-75% in fresh and 25% in
stored samples, while for CP4 EPSPS, it was 38-50% in fresh and 25% in stored
samples.
m
Keywords Milk • Goat • DNA extraction • Transgene
Abbreviations
CP Crude protein
DM Dry matter
GM Genetically modified
s.e.
Solvent extract
R. Tudisco (
*
) • S. Calabr` • M.I. Cutrignelli • M. Grossi • V. Piccolo • F. Infascelli
Dipartimento di Scienze Zootecniche and Ispezione degli alimenti, Facolt` di Medicina
Veterinaria, Universit` degli Studi di Napoli Federico II, Naples, Italy
e-mail:
tudisco@unina.it
