Biology Reference
In-Depth Information
of CALP in canine serum. For this reason, we suppose that the CALP isoform was
the cause of the incorrect subdivision in some electrophoretic patterns. CALP could
create interference with the migration of other isoforms, in particular, BALP. The
Hydragel system is only suitable to measure ALP isoforms in healthy dogs and,
therefore, not applicable in canine clinical practice. It may be interesting to modify
the voltage or contact times between the serum and gel during the automatic phase
of the Hydragel procedure, using a greater number of samples with CALP, to see
whether it is possible to improve the performance of the system.
References
Bottoni C, Ferro E, Salimei E (2003) Electrophoresis using pretreatment of serum with neuramin-
idase and affinity electrophoresis in the evaluation of ALP isoenzymes. Vet Res Commun
27:731-733
Chamberlain BR, Buttery JE, Pannall PR (1992) A simple electrophoretic method for separating
elevated liver and bone alkaline phosphatase isoenzymes in plasma after neuraminidase
treatment. Clin Chim Acta 208:219-224
Feldman EC, Nelson RW (2004) Canine and feline endocrinology and reproduction. Saunders,
St Louis
Gaskill CL, Hoffmann WE, Cribb AE (2004) Serum alkaline phosphatase isoenzyme profiles in
phenobarbital-treated epileptic dogs. Vet Clin Pathol 33:215-222
Hoffmann WE, Dorner JL (1975) A comparison of canine normal hepatic alkaline phosphatase
and variant alkaline phosphatase of serum and liver. Clin Chim Acta 62:137-142
Hoffmann WE, Saneki RK, Dorner JL (1988) A technique for automated quantification of canine
glucocorticoid-induced isoenzyme of alkaline phosphatase. Vet Clin Pathol 17:66-70
Mahaffey EA, Lago MP (1991) Comparison of techniques for quantifying alkaline phosphatase
isoenzymes in canine serum. Vet Clin Pathol 20:51-55
Syakalima M, Takiguchi M, Yasuda J, Hashimoto A (1997a) The age dependent levels of serum
ALP isoenzymes and the diagnostic significance of corticosteroid induced ALP during long-
term glucocorticoid treatment. J Vet Med Sci 59:905-909
Syakalima M, Takiguchi M, Yasuda J, Hashimoto A (1997b) Separation and quantification of
corticosteroid-induced, bone and liver alkaline phosphatase isoenzymes in canine serum.
Zentralbl Veterinarmed A 144:603-10
Syakalima M, Takiguchi M, Yasuda J, Hashimoto A (1998) The canine alkaline phosphatase:
a review of the isoenzymes in serum, analytical methods and their diagnostic application. Jpn J
Vet Res 46:3-11
Teske E, Rothuzen J, de Bruijne JJ, Mol JA (1986) Separation and heat stability of the corticosteroid-
induced and hepatic alkaline phosphatase isoenzymes in canine plasma. J Chromatogr
369:349-356
Van Hoof VO, Van Mullem M, De Broe ME, Lepoutre LG (1993) Comparison of two commer-
cially available systems for electrophoretic separator of alkaline phosphatase isoenzymes.
J Chromatogr 646:235-243
Search WWH ::




Custom Search