Biology Reference
In-Depth Information
test according to Armstrong et al. (
1998
). Nitrates and nitrites were assayed by
means of the Griess reagent (Moncada
1992
). Plasma proteins were determined by
the method according to Lowry et al. (
1951
).
14.3 Results
Table
14.1
shows the activities of antioxidant enzymes. The activities of SOD and
CAT were significantly reduced in adenocarcinoma (43.4% and 74.5%),
in
hemangiopericytoma (
48.0%), and in histiocytoma (14.7% and
17.3%). In contrast, GPx activity was significantly increased in adenocarcinoma
(251.5%) and in hemangiopericytoma (16.3%) but was unchanged in histiocytoma.
Table
14.2
shows the results of the MDA production and nitrite assays. In patients
with adenocarcinoma, the levels of MDA and nitrite significantly increased
with respect to control from 101.1
23.6% and
11.5 to 152.7
18.4 pmol/mg protein and
from 34.5
3.9 to 56.4
6.1 in plasma, and from 107.8
9.7 to 148.4
15.7 pmol/mg protein and 40.7
9.9 in the tissue, respectively.
In subjects with hemangiopericytoma, the MDA and nitrite levels significantly
increased with respect to control from 97.7
4.4 to 86.5
9.9 to 131.9
14.8 and from
31.9
3.6 to 44.8
5.3 in plasma and from 109.7
13.7 to 127.2
13.9 and
from 44.9
2.8 pmol/mg protein in the tissue, respectively. No
significant changes were observed in patients with histiocytoma in terms of MDA
and nitrite production.
5.3 to 105.2
Table 14.1 Antioxidant enzyme activity in cytosol of various tissues (mean
SD)
Samples
SOD % inhibition GPx U/ml
D
%
CAT
m
moles/min/ml
D
%
Control skin (6)
10.6
2.5
0.104
0.04
-
491.1
51.7
-
Histiocytoma (6)
17.3
2.1
0.107
0.03
2.9
418.5
29.8
14.7
Hemangiopericytoma(5) *23.6
2.7
*0.121
0.04
16.3 *255.3
26.3
48.0
Control breast (5)
10.1
2.1
0.097
0.05
-
510.2
47.9
-
Adenocarcinoma (8)
*43.4
4.2
*0.341
0.08 251.5 *129.8
15.3
74.5
*
P
0.01 compared with controls
<
Table 14.2 Nitrite and MDA levels in plasma and cytosol of various tissues (mean
SD)
Samples
MDA (pmol/mg protein)
NO
2
+NO
3
(pmol/mg protein)
Plasma
Cytosol
Plasma
Cytosol
Control skin (6)
97.7
9.9
109.7
13.7
31.9
3.6
44.9
5.3
Histiocytoma (6)
106.4
9.3
104.4
12.6
26.5
5.6
59
6.6
Hemangiopericytoma (5)
*131.9
14.8
*127.2
13.9
**44.8
5.3
**105.2
12.8
Control breast (5)
101.1
11.5
107.8
9.7
34.5
3.9
40.7
4.4
Adenocarcinoma (8)
**152.7
18.4
** 148.4
15.7
**56.4
6.1
**86.5
9.9
*
P
0.05 compared with controls, **
P
0.01 compared with controls
<
<
