Biology Reference
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with the same supplements in a pattern shown in Fig. 3-6. Usually, 100
patches are made, with 50 patches per plate.
Figure 3-6. Patch pattern for 50 patches per plate.
These patches grow out much faster, usually over-night. Each plate is then
replica plated onto other plates, using a velvet stamp slightly smaller than
the surface of the plate. For the original plate with supplements B and C,
four replica plates are used, one with supplement B only, one with C only,
one with no supplement, and the last plate with both supplements. These
plates will score for transductants with the following genetic markers:
plate with supplement B only: A+B+C+, A+B-C+;
plate with supplement C only: A+B+C+, A+B+C-;
plate with no supplement: A+B+C+;
and plate with both supplements: A+B+C+, A+B+C-, A+B-C+, A+B-C-.
Similarly, for the original plate with supplements C and A, also four replica
plates are used:
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