Biology Reference
In-Depth Information
115
FUTURE STUDIES
As illustrated here, we have only discussed about the very simplified kinetic
reaction of a single enzyme. This formulation can be incorporated into the
study of time-dependent kinetics of hemoglobin saturation (see, for
example, Farmery and Roe, 1996), the equilibrium states of which has been
discussed in Chapter 2. It can also be used as a building block for analyzing
metabolic pathways involving a collection of different enzymes (see, for
example, Yang
et al.
, 1999). However, in most cases, only the K
m
and V
max
are of interest to most biochemists and molecular biologists.
Some of the enzymatic reactions are very fast. Special devices have been
developed to measure such reactions in the millisecond range (see, for
example, Cornish-Bowden, 1979). Recently, such measurements are
gradually becoming more routine (see, for example, Sclavi
et al.
, 1998;
Koltermann
et al.
, 1998). Then, the basic mechanism of how enzymes
function at the molecular level can be deciphered.
REFERENCES
Cornish-Bowden A (1979)
Fundamentals of Enzyme Kinetics.
Butterworths London.
Dixon M and Webb EC (1964)
Enzymes.
Academic Press Inc., Publishers, New York.
Farmery AD and Roe PG (1996) A model to describe the rate of oxyhaemoglobin
desaturation during apnoea.
Br. J. Anaesth.,
76
, 284-291
.
Gerald CF (1978)
Applied Numerical Analysis.
Addison-Wesley Publishing Company,
Reading, MA.
Koltermann A, Kettling U, Bieschke J, Winkler T and Eigen M (1998) Rapid assay
processing by integration of dual-color fluorescence cross-correlation spectroscopy: high
throughput screening for enzyme activity.
Proc. Nat. Acad. Sci. USA,
95
, 1421-1426.
McGregor WG, Phillips J and Suelter CH (1974) Purification and kinetic characterization of
a monovalent cation-activated glycerol dehydrogenase from
Aerobacter aerogenes. J. Biol.
Chem.,
249
, 3132-3139.
Michaelis L and Menten ML (1913) Die Kinetik der Invertinwirkung.
Biochem. Z.,
49
, 333-
369.
Sclavi B, Sullivan M, Chance MR, Brenowitz M and Woodson SA (1998) RNA folding at
millisecond intervals by synchrotron hydroxyl radial footprinting.
Science,
279
, 1940-1943.
