Biology Reference
In-Depth Information
87
FUTURE WORK
As pointed out by Rudd (2000),
E. coli
has been an old workhorse in
molecular biology, as well as in biochemistry, cell biology, genetics, etc.
With the complete knowledge of its genome, more intriguing questions can
be asked. There are many open reading frames (ORF's) which can code for
proteins with unknown functions. These proteins can be synthesized
in vitro
and their functions investigated. The development of a new technology
known as DNA chips can thus help our understanding of numerous
biological processes carried out by
E. coli
(Selinger
et al
., 2000). In their
study, they used a 544 X 544 grid of 24 X 24 regions to simultaneously
measure RNA abundance for large numbers of genes under various growth
conditions as a function of time. The information contents of these chips are
extensive. How to analyze such information remains a challenge to both
biologists and mathematicians.
REFERENCES
Bachman BJ (1983) Linkage map of
Escherichia coli
K-12, edition 7.
Microbiol. Rev
.,
47
,
180-230.
Bachmann BJ (1990) Linkage map of
Escherichia coli
K-12, edition 8.
Microbiol. Rev
.,
54
,
130-197.
Bachman BJ, Low KB and Taylor AL (1976) Recalibrated linkage map of
Escherichia coli
K-12.
Bacterial. Rev
.,
40
, 116-167.
Berlyn MKB (1998) Linkage map of
Escherichia coli
K-12, edition 10: the traditional map.
Microbiol. Mol. Biol. Rev
.,
62
, 814-984.
Blattner FR, Plunkett G, Bloch CA, PernaNT, Burland V, Riley M, Collado-Vides J, Glasner
JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ,
Mau B and Shao Y (1997) The complete genome sequence of
Escherichia coli
K-12.
Science,
277
, 1453-1462.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M,
Kitakawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S,
Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Sato N, Sampei G, Seki Y, Sivasunddaram
S, Tagami H, Takeda J, Takemoto K, Wada C, Yamamoto Y and Horiuchi T (1996) A 460-
kb DNA sequence of
Escherichia coli
K-12 genome corresponding to the 40.1-50.0 min
region of the linkage map.
DNA Res
.,
3
, 379-392.
Katinka M, Cossart P, Sibilli L, Saint-Girons I, Chalvignac MA, Le Bras G, Cohen GN and
Yaniv M (1980) Nucleotide sequence of the
thr A
gene of
Escherichia coli. Proc. Nat. Acad.
Sci. USA,
77
, 5730-5733.
