Biology Reference
In-Depth Information
Pollen was viewed with a Nikon TE2000-E inverted microscope and C1 confo-
cal system using Melles Griot Argon Ion (emission 488 nm) and Melles Griot
Helium-Neon (emission 543 nm) lasers, detection filters for GFP and RFP, and
EZ-C1 control and imaging software.
Acknowledgements
We thank Sheila McCormick and Peter Doerner for GEX2-GFP and pCDG
marker lines, Javier Palatnik for mDUO1 cDNA and Nam-Hai Chua for the
estradiol inducible promoter. We acknowledge Prof. Tsuneyoshi Kuroiwa for sup-
porting TM in his work with GCS1::GFP.
Authors' Contributions
Conceived and designed the experiments: LB SH MB DT. Performed the experi-
ments: LB SH MB AS. Analyzed the data: LB SH MB DT. Contributed reagents/
materials/analysis tools: TM DT. Wrote the paper: LB DT.
References
1. McCormick S (2004) Control of male gametophyte development. Plant Cell
16: SupplS142-53.
2. Honys D, Oh SA, Twell D (2006) Pollen development, a genetic and transcrip-
tomic view. In: Malho R, editor. The Pollen Tube: A Cellular and Molecular
Perspective. 3: 15-45.
3. Strome S, Lehmann R (2007) Germ versus soma decisions: lessons from flies
and worms. Science 316: 392-393.
4. Borges F, Gomes G, Gardner R, Moreno N, McCormick S, et al. (2008) Com-
parative Transcriptomics of Arabidopsis Sperm Cells. Plant Physiol 148: 1168-
1181.
5. Engel ML, Chaboud A, Dumas C, McCormick S (2003) Sperm cells of Zea
mays have a complex complement of mRNAs. Plant J 34: 697-707.
6. Okada T, Bhalla PL, Singh MB (2006) Expressed sequence tag analysis of Lili-
um longiflorum generative cells. Plant Cell Physiol 47: 698-705.
7. Okada T, Endo M, Singh MB, Bhalla PL (2005) Analysis of the histone H3
gene family in Arabidopsis and identification of the male-gamete-specific vari-
ant AtMGH3. Plant J 44: 557-568.
