Biology Reference
In-Depth Information
Table 1.
(Continued)
Greenhouse Study
The species included in this study were chosen if there was a known breeding
system, if germinable seeds were available, and if a complementary species (i.e. in
the same family with the opposite breeding system) was known and could also be
obtained as germinable seeds. Seeds were obtained from a variety of sources; Her-
biseed, Rancho Santa Ana Botanic Gardens, Chiltern Seeds, S&S Seeds, and the
National Plant Germplasm System. Our search lead to an unbiased sample of 25
candidate species, allowing five pair-wise family comparisons and four pair-wise
genus comparisons (Table 2).
Table 2.
List of species, with breeding system (O - outcrosser; S - selfer), used in the greenhouse study.
Most species were germinated in 15 cm pots filled to 3 cm below the top with
standard potting soil (Promix BX©). Pots were placed in a greenhouse and wa-
tered daily until the appearance of their first true leaves. Subsequently, they were
watered uniformly every second day to ensure that the soil was kept moist. Some
