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used to analyze the expression difference of these transcripts in floral tissue from
O. dinghushanensis. A P value less than 0.05 was taken to indicate statistical
significance.
Authors' Contributions
CFS and QBL isolated OpdCYC and OpdcyclinD3 genes and performed the
laboratory work of in situ hybridization and gene-specific RT-PCR. RHL carried
out the phylogenetic analyses of OpdCYC and OpdcyclinD3 genes. YZW con-
ceived of and designed the studies, and CFS and QBL participated in the design.
YZW drafted the manuscript and all the authors participated in the editing of the
manuscript. All the authors read and approved the final manuscript.
Acknowledgements
We thank Dr. James Smith for his critical comments and language improvement
on the manuscript. This work was supported by CAS Grant KSCX2-YW-R-135
and National Natural Science Foundation of China Grant, no.30770147.
References
1. Cubas P: Floral zygomorphy, the recurring evolution of a successful trait. BioEs-
says 2004, 26:1175-1184.
2. Endress PK: Antirrhinum and Asteridae - evolutionary changes of floral sym-
metry. Symposium Series of the Society of Experimental Biology 1998, 51:133-
140.
3. Preston JC, Hileman LC: Developmental genetics of floral symmetry evolution.
Trends in Plant Science 2009, 14(3):147-154.
4. Luo D, Carpenter R, Vincent C, Copsey L, Coen E: Origin of floral asymmetry
in Antirrhinum Nature 1996, 383:794-799.
5. Luo D, Carpenter R, Copsey L, Vincent C, Clark J, Coen E: Control of organ
asymmetry in flowers of Antirrhinum Cell 1999, 99:367-376.
6. Gaudin V, Lunness PA, Fobert PR, Towers M, Riou-Khamlichi C, Murray JA,
Coen E, Doonan JH: The expression of D-cyclin genes defines distinct devel-
opmental zones in snapdragon apical meristems and is locally regulated by the
Cycloidea gene. Plant Physiology 2000, 122:1137-1148.
