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and GA2ox1 (GA-up gene) showed that JA treatment did not obviously change
the expression patterns of these three GA-response genes in the ga1-3 gai-t6 rga-
t2 rgl1-1 quadruple mutant (Figure 5C). These data suggested that JA signaling
might mediate a specific branch of GA signaling to regulate the expression of the
three MYB genes.
Figure 6.
GA Regulates the Expression of JA Biosynthesis Genes DAD1 and LOX1.
(A) RT-PCR
analysis of GA- and JA-biosynthesis genes in the young flower buds of La-er WT control, ga1-3,
ga1-3 gai-t6 rga-t2 rgl1-1 (Q3), ga1-3 gai-t6 rga-t2 rgl1-1rgl2-1 (penta), opr3 (in WS background) and Ws
WT control. (B) Northern analysis of MYB21, GA20ox2, LOX1, LOX2 and OPR3 in the young flower buds
of the La-er WT control, ga1-3, Q3, penta, opr3 and Ws WT control. (C) Semi-quantitative analysis of DAD1
expression in ga1-3, Q3 and penta relative to that in WT (Laer), respectively. Data were averaged from three
independent batches of samples and ACTII was used as the normalization control. The expression level of WT
is set as 1. (D) Comparison of JA contents among WT (La-er), opr3, ga1-3 gai-t6 rga-t2 rgl1-1 (Q3) and ga1-3
gai-t6 rga-t2 rgl2-1 rgl1-1 (penta). For WT and the penta mutant JA contents were averaged from four repeats.
For the Q3 mutant, JA was detected in three out of the four repeats. For opr3, JA was detected only in one out
of the four repeats. FW, fresh weight.
