Biology Reference
In-Depth Information
phenotype in the hepta mutant was due to reduced cell length (Figure 4B and
4C), rather than to a reduction in cell number (Figure 4D). Therefore, MYB21
and MYB24 act downstream of DELLAs in GA signaling pathway to control the
stamen filament development.
Figure 4.
myb21-t1 myb24-t1 Is Epistatic to ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 in Controlling Stamen Filament
Elongation.
(A) Comparison
of the stamen phenotype among ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1(penta), ga1-3 gai-t6 rga-t2
rgl1-1 rgl2-1 myb21-t1 myb24-t1 (hepta), ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 myb21-t1 (hexa1) and ga1-3 gai-t6
rga-t2 rgl1-1 rgl2-1 myb24-t1 (hexa2). (B) SEM of stamen filament epidermal cells in the penta, hepta, hexa1
and hexa2 mutants. Segments shown were all from the middle part of the filament. Some individual cells were
outlined with black lines for easy visualization. (C) Comparison of stamen and pistil lengths among different
genotypes. Filament and pistil lengths were measured from SEM pictures (n = 30). (D) Average number of
epidermal cells per stamen filament in penta, hepta, hexa1 and hexa2. n: number of stamens used in counting.
