Biology Reference
In-Depth Information
reduction in only 50% of the seeds produced by wild type ovules crossed to
met1-3/+ pollen. Accordingly, we observed both large and small seeds by visual
inspection (Figure 1A; 45.4% small seeds; n = 900) and quantitative analysis
(wt×met1/+, n = 374; wt×wt, n = 257; P<.0001 for ANOVA, t-test and Mann
Whitney) (Figure 1C, Table 1). In the small seeds from crosses between wild type
ovules and pollen from met1-3/+ plants, embryo growth was relatively normal as
compared to the endosperm, which exhibited reduced growth.
Figure 1.
Parental effect of met1-3/+ on seed size.
(A) Seed
populations produced by crosses between wild-type (wt) ovules and pollen from met1-3/+ or met1-3/
met1-3 plants. The scale bar represents 0.5 mm. (B) Morphometric parameters of seeds from crosses between
wt ovules and pollen from wt or from met1/met1 plants. (C) Morphometric parameters of seeds from crosses
between ovules from wt plants and met1-3/+ pollen. The green and red ovals represent the extent of the
populations of seeds shown in B. (D) Morphometric parameters of seeds from crosses between ovules from
met1/+ plants and wild-type pollen. The green and red ovals represent the extent of the populations of seeds
shown in B.
Table 1.
Morphometric measurements of seeds from various crosses reported in Figure 1.