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On the other hand, on medium containing ABA, the expression levels of
ABI5, ABI3, Em1 and Em6 were similar in the wild type and wrky2 mutants
at 4 days post-stratification. However, at 7 days post-stratification, there were
higher expression levels of these genes in wrky2 mutants than in the wild type,
which was consistent with microarray analysis (Figure 6 and Table 1). By contrast
at 4 days post-stratification, the expression of ABI3 and Em6 didn't decrease or
decreased only slightly in wrky2 mutants, but decreased rapidly in the wild type
within 7 days post-stratification. Within 7 days post-stratification, expression of
AIB5 decreased faster in wild type than in wrky2 mutants. Expression of Em1
increased in wrky2 mutants 7 days post-stratification, but was unchanged in wild
type (Figure 6).
Table 1. Microarray analysis of wrky2 mutants and wild type
These results indicated that wrky2 mutants displayed delayed or decreased
expression of ABI5 and ABI3, but increased or prolonged expression of Em1 and
Em6.
The Expression of WRKY2 in ABA-Insensitive Mutants and
ABA-Deficient Mutants
Because wrky2 mutants are more sensitive to ABA during seed germination and
postgermination growth arrest than the wild type, and wrky2 mutants also affect
important genes of the ABA signal pathway in the regulation of germination and
postgermination growth, we analyze whether abi3-1, abi5-1, aba2-3 and aba3-1
mutants have an effect on the expression of WRKY2. We germinated all seeds on
MS medium with 0 or 1.5 ยต M ABA for 4 days post-stratification. We performed
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