Biology Reference
In-Depth Information
reactivated by ABA during a short development window. Given the ABA hyper-
sensitivity of wrky2 mutants, we studied the effect of ABA on the early develop-
ment of wrky2 mutants. Application of 5
µ
M ABA [5] within 48 h post-stratifi-
cation maintained the germinated embryos of wrky2 mutants in an arrested state
for several days, but did not prevent germination, whereas a significant percentage
of wild-type germinated embryos escaped growth arrest and turned green (Figure
5A and 5B). ABA applied outside the 48-h time frame failed to arrest growth and
prevent greening (Figure 5C). If the seeds were transferred to ABA-containing
media immediately or one day after stratification, we observed that the wrky2
mutants were more sensitive to ABA responses than the wild type. However, if the
seeds were transferred to ABA-containing media 2 days post-stratification, there
was no significant difference between the wild type and wrky2 mutants. These
results indicated that wrky2 mutants were hypersensitive to ABA responses for a
short development window.
Figure 5.
wrky2 mutants are hypersensitive to ABA responses in a short development window.
(A) Seeds
were germinated on MS medium 1 d after stratification, all were transferred to MS medium with 5
µ
M ABA. Photographs were taken 6 d after transfer. (B) Seeds were germinated on MS medium and 2 d after
stratification, all were transferred to MS medium with 5
µ
M ABA. Photographs were taken 5 d after transfer.
(C) Seeds were germinated on MS medium and 3 d after stratification, all were transferred to MS medium with
5
µ
M ABA. Photographs were taken 4 d after transfer.
