Applications of Raman and Surface-Enhanced Raman Scattering to the Analysis of Eukaryotic Samples - Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields - page 82

Fig. 4.4. Influence of carotenoid pre-resonant Raman contributions on cluster anal-
ysis result. The dendrogram was obtained using the same parameters as in the one
displayed in Fig. 4.3. However, the spectra of the used data set were obtained with-
out decomposing carotenoid before the Raman experiments. The species sallow,
horse-chestnut and large-leaved linden form a distinct cluster due to the intense
carotenoid contribution. The corresponding spectra are shown in Fig. 4.5 traces a,
c and e. Reprinted with permission from [52]

In particular, the spectra of large-leaved linden, sallow and horse-chestnut
are dominated by typical carotenoid features (compare spectra a, c and e
of Fig. 4.5). These spectra display the characteristic combination of the
intense carotenoid vibrations

ν 1 ∼ 1520 cm − 1 , ν 3 ∼ 1160 cm − 1 and

ν 6

∼

1006 cm − 1 and also of the much weaker modes ν 2 ∼ 1190 cm − 1 and in

1280 cm − 1 [70, 71]. In the other investigated tree species,
such intense carotenoid features were not detected and/or probably super-

ν 5 ∼

some cases