Biomedical Engineering Reference
In-Depth Information
Fig. 14.4.
Decreased mineral content is seen at osteogenic fronts (OFs) of
the loaded specimen and increased matrix production extends beyond the OFs.
Increased matrix is present on the endocranial side of the tissue of both loaded and
unloaded specimens. Raman score images and RGB images for 4-day loaded and un-
loaded specimens. The endocranial surfaces of the calvaria are at the bottom of the
images. (
A-G
) Loaded specimen. (
H
-
I
) Unloaded specimen. (
A
,
E
,and
H
)Mineral
score image. (
B
,
F
,and
I
) Matrix score image. Scale for Raman score images:
red
,
high intensity;
blue
, low intensity. (
C
,
G
,and
J
) RGB contrast-enhanced images
(
blue
areas indicate mineral presence;
green
areas indicate matrix presence;
light blue-
green
areas indicate the presence of mineral and matrix). (
D
) Reference hematoxylin
and eosin-stained section from the same specimen imaged in
A
-
G
.The boxed area
of the specimen shown in
A
-
D
was reimaged to show the OF on a smaller intensity
scale and show more detail; the Raman score images are shown in
E
-
G
. The loaded
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