Coherent Raman Scattering Microscopy - Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields - page 141

Fig. 6.15. Multiplex stimulated Raman loss microspectroscopy of 20-μm
polystyrene beads dispersed in water. A Raman spectra retrieved from SRL spectra
of an individual bead and of bulk water at locations indicated by circles and squares,
respectively, in the images. B Reconstructed Raman images of the beads repre-
senting the density maps of three characteristic Raman resonances at 1003 cm − 1 ,
2904 cm − 1 , and 3066 cm − 1 of polystyrene (Courtesy of Evelyn Ploetz et al., after

[21])

significantly reduced laser pulse energies, future technological improvements
and optimization of the SRL detection sensitivity are required.

6.5 Conclusions and Perspectives

A series of advances over the past decade have made CRS microscopy a highly
sensitive tool for label-free imaging and vibrational microspectroscopy that is
capable of real-time, non-perturbative studies of complex biological samples
based on molecular Raman spectroscopy. In particular, biomedical applica-
tions where fluorescent labeling of small molecules represents a severe pertur-