Fluorescence Reporting Based on FRET Between Conjugated Polyelectrolyte and Organic Dye for Biosensor Applications - Advanced Fluorescence Reporters in Chemistry and Biology - page 444

Scheme 13 Illustration of the specific detection of target proteins by using the complex of a
cationic polythiophene(21)/dye-labeled ssDNA aptamer on glass slides. Reproduced with permis-
sion from [ 102 ]
be established by taking advantage of CPEs in association with dye-labeled apta-
mers, which opens new feasibilities for simple and rapid multiplex analysis in
proteomics.
3.3 CPE Complex Based Sensor
In addition to protein detection using specific antibody/antigen and aptamer/protein
interactions, array detection was demonstrated based on nonspecific interactions
between the CPE/dye-labeled ssDNA complexes and proteins [ 103 ]. The design
concept is motivated by the fact that external agents can effectively perturb the
electron coupling of optical units within the complex of CPE/ssDNA-C* and in turn
vary the FRET-induced fluorescence of both CPE (donor) and C* (acceptor).
Owing to discrepancies in local hydrophobic and charged domains of different