Optical Reporting by Conjugated Polymers via Conformational Changes - Advanced Fluorescence Reporters in Chemistry and Biology - page 403

Fig. 12 (a) Photos of wells labeled in graph B exhibiting colorimetric changes. (b) UV-vis
absorption spectra of polymer 1 (2.9 10 9 mol on a monomer unit basis) in the presence

10 10 mol of the 15-mer) and different salts in 100 of X1 DNA aptamer (1.9 m l of water at

25 C[ 24 ]

Fig. 13 Fluorescent spectra
of (a) polymer 1, (b) human
thrombin/X1 aptamer/
polymer 1, (c) human
thrombin/X2 aptamer/
polymer 1 mixture, and (d)
X1/polymer 1 complex in
water, measured at 50 C[ 24 ]

in fluorescence intensity based upon complexes with specific and nonspecific apta-
mers and the polymer. These differences in fluorescence intensity are most likely due
to quenching that occurs as the polymer in the planar form tends to aggregate, causing
a lower fluorescence intensity due to intra-chain de-excitation events [ 25 ]. The
a

-thrombin was able to be detected at a concentration of 10 pM in 200 m l.