Chemistry Reference
In-Depth Information
Fig. 4 (a) The 500-MHz
NMR spectrum of human
113
a
I
V
Cd
7
-MT3 in 15 mM
phosphate buffer, pH 7.3 and
310 K. Resonances I, V, VI,
and VII are assigned to the
a
-domain and II, III, and IV to
the
b
-domain [
27
]. (b)2D
113
VI
VII
II
III
IV
Cd-
113
Cd COSY NMR of
Cd
7
-MT10 in 17 mM Tris-d
11
buffer, pH 7.0, 298 K with the
one-dimensional proton-
decoupled
113
Cd NMR
spectrum at the
top
. The
cadmium connectivity in the
a
700
680
660
640
620
600
580
113
Cd(ppm)
II
I
b
III IV
VI
VII
V
-domains is also
shown [
81
]. (c) The 500 MHz
two-dimensional
1
H-
113
Cd
HMQC spectrum of human
113
Cd
7
-MT3 in 15 mM
phosphate buffer with
3
J
(
1
H,
113
Cd) of 30 Hz. The
assignments were also labeled
with one-letter amino acid
code [
27
]
- and
b
ppm
α
463.5
460
α−
cluster VII
500
β
521.1
VI
β−
cluster
540
α
571.8
V
580
620
α
642.1
652.1
669.4
673.3
α
IV
β
660
III
β
II
I
650
600
550
500
ppm
c
C38
C37
C37
C51
600
C42
C67
C67
C45
620
C35
C38
C34
C45
C35
C34
C49
640
660
C67
C66
C64
C64
C51
C51
C67
680
4.0
3.6
3.2
2.8
2.4
1
H (ppm)
