Chemistry Reference
In-Depth Information
Table 2 (continued)
Method
a
Temp
C
Structure class
Protein
Size,
kDa
b
#ofTM
a
Detergent
Detergent:
protein molar
ratio
PDB ID Ref
)
c
(
b
QseC
h
21
2
LMPG
330
45.37
2KSE
[
88
]
KdpD
h
11
4
LMPG
330
45.37
2KSF
[
88
]
Presenilin
catalytic
fragment
19
3
SDS
>
140
40
2KR6
[
136
]
DAGK trimer
i
43
3
3
DPC
-
45
2KDC
[
38
]
UCP2
j
33
6
DPC/DMPC/
CL
(150:2:1)
190
33
2LCK
[
41
]
a
Method used to determine structures are divided into seven different classes, depending on the type of data and experiments that were used for the structure
calculation
b
Total molecular weight for all protein subunits in the complex
c
Number of
b
-strands spanning the membrane are denoted in parentheses
d
Standard NOE-based structure determination protocols typically applied to small water-soluble globular proteins
e
Selected representatives
f
Positioning of amphipathic helices in this structure represents ensemble average on the timescale of RDC and NOE restraints
g
PREs were used to localized solvent-exposed or micelle-bound residues
h
No NOE restraints directly used, but hydrogen bond restraints were imposed for the TM helices
i
Data from disulfide bond mapping was used to create intermolecular restraints
j
No NOE data used