Chemistry Reference
In-Depth Information
3 Membrane Mimetic Systems for Solution NMR ........................................... 129
3.1 Micelles ................................................................................ 129
3.2 Bicelles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
3.3 New Developments in Membrane-Mimetic Solvents for Solution NMR ........... 142
3.4 Approaches for Sample Screening ................................................... 146
4 Methods for Acquisition of Solution NMR Spectra of Membrane Proteins .............. 147
4.1 Isotope Labeling Schemes for Large Protein Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
4.2 Relaxation Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
5 Strategies for Membrane Protein Structure Determination by Solution NMR . . . . . . . . . . . 154
5.1 NOE-Based Methods of Structure Determination ................................... 154
5.2 Non-uniform Sampling . . ............................................................. 155
5.3 Global Folds in the Absence of Long-Range NOEs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
6 Concluding Remarks . ....................................................................... 164
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Abbreviations
b
-Octyl glucoside
14-
O
-PC 1,2-Di-
O
-tetradecyl-
sn
-glycero-3-phosphocholine
6-
O
-PC 1,2-Di-
O
-hexyl-
sn
-glycero-3-phosphocholine
C
6
-DHPC 1,2-Dihexanoyl-
sn
-glycero-3-phosphocholine
C
7
-DHPC 1,2-Diheptanoyl-
sn
-glycero-3-phosphocholine
CF
-OG
b
Cell-free
CHAPS
3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
COSY
Correlation spectroscopy
CSA
Chemical shift anisotropy
CTAB
Cetyl-trimethylammonium bromide
DAGK
Diacylglycerol kinase
DDM
Dodecylmaltoside
DHAP
Dihexadecyldimethylammonium bromide
DPC
Dodecylphosphocholine
DTAB
Dodecyl-trimethylammonium bromide
EPR
Electron paramagnetic resonance
gA
Gramicidin A
GpA
Glycophorin A
GPCR
G-protein coupled receptor
HSQC
Heteronuclear single quantum coherence
LDAO
Lauryldimethylamine-oxide
LMPC
Lyso-myristoyl phosphatidylcholine
LMPG
Lyso-myristoyl phosphatidylglycerol
LPPG
Lyso-palmitoyl phosphatidylglycerol
MDD
Multidimensional decomposition
MSP
Membrane scaffold protein
MTSL
1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl-
methanethiosulfonate
NMR
Nuclear magnetic resonance
