Biology Reference
In-Depth Information
Figure 3.
Effect of temperature on activity of recombinant
Arthrobacter
sp. 32c
β
-D-galactosidase at
pH range from 4.5 to 9.5.
Table 2.
Effects of metal ions on
Arthrobacter
sp. 32c
β
-D-galactosidase activity.
Metal ion
Relative activity [%]
None
100
Na
+
97 ± 3
K
+
100 ± 2
Ni
2+
38 ± 4
Mg
2+
90 ± 2
Fe
2+
25 ± 2
Co
2+
87 ± 3
Cu
2+
0±0
Mn
2+
11± 2
Zn
2+
0±0
Ca
2+
88 ± 2
Table 3.
Effects of thiol compounds on recombinant
Arthrobacter
sp. 32c
β
-D-galactosidase activity.
Compound
Relative activity [%]
None
100
2-mercaptoethanol
92 ± 4
DTT
96 ± 2
Glutathione reduced
6 ± 3
L-cystein
95 ± 2