Biology Reference
In-Depth Information
Figure 3. Effect of temperature on activity of recombinant Arthrobacter sp. 32c
β
-D-galactosidase at
pH range from 4.5 to 9.5.
Table 2. Effects of metal ions on Arthrobacter sp. 32c
β
-D-galactosidase activity.
Metal ion
Relative activity [%]
None
100
Na +
97 ± 3
K +
100 ± 2
Ni 2+
38 ± 4
Mg 2+
90 ± 2
Fe 2+
25 ± 2
Co 2+
87 ± 3
Cu 2+
0±0
Mn 2+
11± 2
Zn 2+
0±0
Ca 2+
88 ± 2
Table 3. Effects of thiol compounds on recombinant Arthrobacter sp. 32c
β
-D-galactosidase activity.
Compound
Relative activity [%]
None
100
2-mercaptoethanol
92 ± 4
DTT
96 ± 2
Glutathione reduced
6 ± 3
L-cystein
95 ± 2
 
Search WWH ::




Custom Search