Biology Reference
In-Depth Information
possibly increasing AcCoA production, some of which constrain growth more than
others (see Figures 6A and B).
Figure 6.
Mutational strategies for increased PHA production.
This figure highlights six strategies
suggested by the modified Optknock approach for increased production of AcCoA, a precursor for
polyhydroxyalkanoates. (A) The AcCoA production ranges versus growth yield of
in silico
strains
developed using the “AcCoA production” strategy. (B) The AcCoA pooling versus growth yield of
in
silico
strains developed using the “AcCoA pooling” strategy.
One promising hypothesis (strategy 2) generated by the AcCoA production meth-
od predicted that a double-mutant devoid of 6-phosphogluconolactonase (pgl/PP1023)
and periplasmatic glucose dehydrogenase (gcd/PP1444), would produce 29% more
AcCoA than the wild type growing on glucose as a carbon source (Figure 6A). As we
are currently still in the process of generating this mutant, we were not yet able to test
the prediction. Another promising hypothesis (strategy 1) included knocking-out tri-
ose phosphate isomerase (tpiA/PP4715). As the mutant for tpiA was generated in this
