Biology Reference
In-Depth Information
non-redundant set of Genbank proteins for phylogenetic analysis. The BssABC, pres-
ent in
A. aromaticum
EbN1 [20, 22] as well as
Thauera aromatica
[6], and
Geobacter
metallireducens
[23], is not present in either the
D. aromatica
or
Azoarcus
BH72 ge-
nomes (see Table 1). The BcrAB and BssABC, previously denoted as “central” to an-
aerobic catabolism of aromatics, are likewise absent. The set of recruited proteins for
both BssABC and BcrAB indicate they are not as universally present as has been sug-
gested.
Dechloromonas
aromatica
does encode a protein in the pyruvate formate lyase
family, but further analysis shows that it is more closely related to the
E. coli
homolog
of this protein (which is not involved in aromatic catabolism) than to BssA. Anaerobic
reduction of ethylbenzene is carried out by ethylbenzene dehydrogenase (EbdABCD1,
2) in
A. aromaticum
. This complex belongs to the membrane-bound nitrate reductase
(NarDKGHJI) family. In
D. aromatica
, this complex of proteins is only present as
the enzymatically characterized perchlorate reductase (PcrABCD; [24]) which utilizes
perchlorate, rather than nitrate, as the electron acceptor. The EbdABCD proteins in
A.
aromaticum
(VIMSS814904-814907 and VIMSS816928-816931) occur in operons
that include (S)-1-phenylethanol dehydrogenases (Ped; VIMSS814903 and 816927)
[25], both of which are absent from
D. aromatica
, as is the acetophenone carboxylase
that catalyzes ATP-dependent carboxylation of acetophonenone produced by Ped.
Table 1.
Anaerobic aromatic degradation enzymes in near-neighbor
Aromatoleum aromaticum
EbN1.
Proteins involved in the
anaerobic aromatic pathways
in Aromatoleum aromaticum
str. EbN I
A. aromaticum EbN I - repre-
sentative protein used for HMM
models
Azoarcus
BH72
ortholog
D. aromatica RCB
ortholog
I) phenylalanine
Pat
VIMSS813888:pat
(COG 1448; EC 2.6. 1.57)
-
-
Pdc
VIMSS817385:pdc (COG3961)
-
-
Pdh
VIMSS816687:pdh (COG I 0 12)
-
-
lorAB
VIMSS813644:iorA (COG4321)
-
+
2) phenylacetate
Pad BCD
VIMSS816693:padB
-
-
PadEFGHI
VIMSS816700:padl
-
-
Padj
VIMSS81670 I :padj
-
-
3) benzyl alcohol/benzaldehyde
Adh
VIMSS815388:adh (COG I 062)
-
-
Ald
VIMSS816847:ald
(COG 1012; ECI .2.1.28)
+
-
4) p-eresol
PehCF
VIMSS813733:pchC (EC:
1.17.99. 1)
-
-
PehA
VIMSS815385:pchC
-
-
VIMSS813734:pchF (EC1.1.3.38)
-
-
VIMSS815387:pchF
-
-
