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and BSA-Al groups. Immunization with either empty ARC-
BM
/
GC
failed to evoke
any anti-BSA IgG response, whereas only one out of four mice receiving adjuvant-
free BSA displayed detectable specific antibodies. Mean titers in ARC-
BM
/
GC
-BSA
groups were sustained until at least day 60 and declined by day 135. In all cases, very
little anti-BSA IgM reactivity was detected throughout the study (data not shown).
Figure 5.
Humoral response to ARC-entrapped BSA. (A) Primary antibody response. Mice were
immunized sc on days 0 and 21 with 25 μg of BSA alone, BSA adsorbed on Al
2
O
3
, BSA entrapped
in TEB-
BM
and TEB-
GC
or empty TEB. Serum samples were collected on days 28 and 60, and ELISA
assayed for anti-BSA antibody titers. (B) Long-term memory response to ARC-entrapped BSA. Mice
were immunized sc on days 0 and 21 with 25 μg of BSA alone, BSA adsorbed on Al
2
O
3
or BSA
entrapped in ARC, and boosted on day 180 with 25 μg of BSA (without adjuvant). Serum samples
were collected 3 weeks later and assayed for anti-BSA antibody titers. Values represent mean titers ±
SEM. ANOVA, *
p
< 0.05, **
p
< 0.01.