Biology Reference
In-Depth Information
Time- and Concentration-dependent Growth Curve
While several compounds identified in our study could be used as excellent drug leads
in vitro
, the best and most valuable ways would be
in vivo
validation. The follow-
ing results of the time- and concentration-dependent effects of the lead inhibitors on
the growth of
S. pneumoniae
further illustrated their antibacterial characteristics, and
would be an important guide for
in vivo
administration. As shown in Figure 6, the sim-
ilar curves of compounds 1, 2, 3, and 5 indicated that these compounds have signifi-
cant activity against
S. pneumoniae
at concentration of about 200 μM, and this activity
could last at least 8 hr. The most efficient inhibitor identified was compound 6, which
had bactericidal effect against
S. pneumoniae
even at concentration of as low as 0.2
μM. However, even at concentration of 400 μM, compound 4 was not likely to have
bactericidal effect, but it seemed to have delayed the multiplication of
S. pneumoniae
.
Figure 6.
Time and concentration-dependent effects of the candidate compounds on the growth of
S. pneumoniae
in
vitro
.
